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New Single Cell Sequencing instruments from Mission Bio available to use free of charge

Expanding our single-cell offerings, users can now access Mission Bio's technologi at the NSC Ullevål node. The Tapestri instrument enables targeted re-sequencing of single cells, such as detection of SNPs and CNVs at the single-cell level. Applications include characterising tumour heterogeneity , checking off-target hits in genome-editing, or measuring levels of mosaicism. Single-cell DNA methylation analysis is in development. These capabilities can be combined with oligo-tagged antibodies to detect the co-expression of proteins with SNPs / CNVs (single-cell multi-omics). The machine is available for use free of charge, as long as you perform the sequencing with us afterwards! For more information, see https://missionbio.com/, and contact us on post@sequencing.uio.no for an introduction and to reserve the machine.

 

After the digital PacBio Day in 2021, we are happy to announce that this year we plan for in-person event in the campus! We will also expand it to a full-day event and include human research.

Stay tuned for more information and registration later this summer!

The NSC will close over summer. Deadline for submissions is 3 pm on Thursday 30th June. Projects queued before this deadline will still be processed over summer, but longer turnaround times should be expected. We reopen again from Monday 1st August.

The NSC will close over Easter. Deadline for submissions is 3 pm on Tuesday 12th of April. We will reopen again from Tuesday 19th April.

God Påske!

The NSC will close over the Christmas break. Deadline for submissions is 4 pm on Wednesday 22nd December. We will reopen again from Monday 3rd January 2022.

God Jul!

Next Generation Cytogenomics has also arrived at the NSC! Optical mapping is a complementary technology to sequencing that enables improved genome assembly and the detection of structural variants (indels, translocations and CNVs) that sequencing struggles to detect. With high coverage of the genome, even low-frequency rearrangements in tumour samples or genetic mosaics can be detected.

 

For early access prior to service launch, or just to learn more, get in touch (post@sequencing.uio.no)!

 

More info at https://bionanogenomics.com

The NSC will be closed in July. The last day for submissions will be Friday 2nd July. We will re-open for submission from August 2nd (OUS) and August 9th (UiO). Projects submitted prior to July 2nd will still be processed over the summer, but turnaround times will be longer. Consulting / advice will only resume when we reopen.

After 5 years of service, we have retired our HiSeq 3000, 4000 and X instruments.  These are superceded by the newer, faster, cheaper NovaSeq. From now, Illumina sequencing will be performed only at the OUS node, and the CEES node will be focusing on long-read PacBio services.

The NSC will close over Easter. Deadline for submissions is 12.00 noon on Wed 31st March. We will reopen again from Tuesday 6th April.

God Påske!

The NSC will close over the Christmas break. Deadline for submissions is 15:00 on Friday 18th December. We will reopen again from Monday 4th January 2021.

God Jul!

We will not be closing over the summer (both UiO and OUS nodes). Depending on demand, we may offer a reduced service. During the month of July, please contact us in advance of making a delivery to ensure we are available to receive your samples on post@sequencing.uio.no

Both the UiO and OUS nodes are open to receive and sequence your samples.

We are not experiencing sickness or shortage of reagents and remain open. However, due to distancing requirements, we have fewer employees on site at any one time, so please ensure we are available to receive your samples before delivery, by mailing us on post@sequencing.uio.no.

We request that all persons delivering samples show consideration, particularly in the hospital environment, and only deliver samples in person if the are:

1. Themselves free of possible COVID-19 symptoms

2. Have no family members in quarantine

 

Both the UiO and OUS nodes are open to receive and sequence your samples.

We are not experiencing sickness or shortage of reagents and remain open. However, due to distancing requirements, we have fewer employees on site at any one time, so please ensure we are available to receive your samples before delivery, by mailing us on post@sequencing.uio.no.

We request that all persons delivering samples show consideration, particularly in the hospital environment, and only deliver samples in person if the are:

1. Themselves free of possible COVID-19 symptoms

2. Have no family members in quarantine

 

Single Cell Sequencing instruments available to use free of charge

We have a Chromium Controller available at both NSC nodes (Blindern and Ullevål). We are not offering 10X single cell library preparation as a service, but make these machines available to users free of charge, as long as you perform the sequencing with us. Single-cell RNA-seq, single-cell ATAC-seq and "linked"  (synthetic long) reads are among the possible applications. To book a machine, please simply inquire on post@sequencing.uio.no.

More info here: https://www.10xgenomics.com/

Our Illumina NovaSeq 6000 system is now available. The latest submission form is updated with all yield options, which range from 400M reads (the same as a HiSeq lane) to 10 Billion reads per flowcell (equivalent to approx 2.5 HiSeq flowcells). We will be happy to help you choose the most appropriate yield from the somewhat confusingly-named flowcells (SP, S1, S2 and S4)!

Run times are all under 48 hours, and 250 bp paired end reads are back in high output on the NovaSeq.  For more information see: https://emea.illumina.com/systems/sequencing-platforms/novaseq/specifications.html.

Users can expect a small drop in price for genome sequencing, but RNA-seq and counting applications such as ChIP-seq will be 15-40% cheaper than before, depending on scale and readlength.

The NSC will close over the Christmas break. Deadline for submissions is 15:00 on Thursday 19th December. We will reopen again from Monday 6th January 2020.

God Jul!

New 16S Service

To meet demand, we have launched a 16S service. We offer amplification and sequencing with the "Fadrosh et al" procedure (https://doi.org/10.1186/2049-2618-2-6), which gives excellent results with only 10% PhiX blend, with sequencing on the MiSeq with 300 bp paired end reads. The V3-V4 regions are targeted with primers 319F-806R. Prices for sample batches of ≤24, ≤48, ≤94 and ≤190 are 35, 40, 45 and 50,000 NOK (including MVA and a MiSeq run). Users must supply purified genomic DNA, and can choose to receive data in FASTQ, QIIME or MOTHUR analysis formats. For input requirements etc., please see our submission guidelines.

10x Genomics Chromium Controller now available at NSC

Single-cell RNA-seq, single-cell ATAC-seq and "linked"  (synthetic long) reads are among the possible applications. If you are interested in accessing these services, please get in touch, as we are soliciting early-access users! More info here: https://www.10xgenomics.com/

After 6 years as a workhorse of the facility, we are now phasing out HiSeq 2500 services. The OUS HiSeq 2500 has been decommissioned, but the machine at CEES will be available until end of March 2019.

The NSC will not be accepting new submissions over the summer break (sequencing of existing submissions will continue, although turnaround times may be longer than usual). Deadline for submissions is 16:00 on Thursday 5th July. We will reopen again from Monday 6th August.

The NSC will not be accepting submissions over Easter. Deadline for submissions is 12.00 noon on Friday 23rd March. We will reopen again from Tuesday 3rd April.

God Påske!

Renovation of the OUS node is complete, and normal services have resumed.

All four HiSeq X machines are now operational! - output capacity at OUS now exceeds 1.5 Tb per day...

 

Pre and post-PCR labs were expanded, and installation of new sample-prep  automation is underway. However, the biggest change is the installation of a powerful air-conditioning system in our enlarged machine room.Two 20 kW systems (one main system, and an independent backup powered from a reserve generator) should ensure stable temperatures in the machine room, improving machine reliabiliy and data quality.

Ask for a quick tour when you drop off your samples!