Dept. of Medical Genetics Ullevål
Kirkeveien 166 (map)
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The NSC will close over the Christmas break. Deadline for submissions is 15:00 on Thursday 19th December. We will reopen again from Monday 6th January 2020.
The NSC has purchased an Illumina NovaSeq 6000 system, and will make this available to users as a service as soon as installation and testing are completed. A high capacity sequencer, the NovaSeq can generate up to 6 Tb data per run in under 48 hours. Smaller ouput runs are also available - for more information see: https://emea.illumina.com/systems/sequencing-platforms/novaseq/specifications.html. Users can expect a small drop in price for genome sequencing, but RNA-seq may be 15-40% cheaper, depending on scale and readlength.
New 16S Service
To meet demand, we have launched a 16S service. We offer amplification and sequencing with the "Fadrosh et al" procedure (https://doi.org/10.1186/2049-2618-2-6), which gives excellent results with only 10% PhiX blend, with sequencing on the MiSeq with 300 bp paired end reads. The V3-V4 regions are targeted with primers 319F-806R. Prices for sample batches of ≤24, ≤48, ≤94 and ≤190 are 30, 35, 40 and 50,000 NOK (including MVA and a MiSeq run). Users must supply purified genomic DNA, and can choose to receive data in FASTQ, QIIME or MOTHUR analysis formats. For input requirements etc., please see our submission guidelines.
10x Genomics Chromium Controller now available at NSC
Single-cell RNA-seq, single-cell ATAC-seq and "linked" (synthetic long) reads are among the possible applications. If you are interested in accessing these services, please get in touch, as we are soliciting early-access users! More info here: https://www.10xgenomics.com/
After 6 years as a workhorse of the facility, we are now phasing out HiSeq 2500 services. The OUS HiSeq 2500 has been decommissioned, but the machine at CEES will be available until end of March 2019.
The NSC will close over the Christmas period. The last day for submissions in 2018 will be Wed 19th December. We will reopen again from Thursday 3rd January.
Users of NorSeq sequencing core facilities (includes both nodes of the NSC - for full details of participating facilities please see https://www.norseq.org/) were overwhelmingly satisfied with the services provided! For a full summary of answers provided in response to our 2018 user survey, please see the following document: https://www.sequencing.uio.no/news/2018/norseq-user-survey-2018.pdf
The NSC will not be accepting new submissions over the summer break (sequencing of existing submissions will continue, although turnaround times may be longer than usual). Deadline for submissions is 16:00 on Thursday 5th July. We will reopen again from Monday 6th August.
The NSC will not be accepting submissions over Easter. Deadline for submissions is 12.00 noon on Friday 23rd March. We will reopen again from Tuesday 3rd April.
Renovation of the OUS node is complete, and normal services have resumed.
All four HiSeq X machines are now operational! - output capacity at OUS now exceeds 1.5 Tb per day...
Pre and post-PCR labs were expanded, and installation of new sample-prep automation is underway. However, the biggest change is the installation of a powerful air-conditioning system in our enlarged machine room.Two 20 kW systems (one main system, and an independent backup powered from a reserve generator) should ensure stable temperatures in the machine room, improving machine reliabiliy and data quality.
Ask for a quick tour when you drop off your samples!
Illumina’s lastest sequencers, HiSeq X, HiSeq 4000 and HiSeq 3000 have now been purchased and are being installed at the NSC.
HiSeq X is targeted at population-scale genome sequencing and NSC now has 4 of these machines. The first has been installed and tested. We expect the remaining 3 machines to be operational before summer.
HiSeq 3000 and 4000 build upon the existing HiSeq 2500 platform using the new HiSeq X patterned flow cell technology. The principle benefit to users will be faster turnaround time (run time is 3 days compared to 6 days in HiSeq 2500). Also, the new patterned flow cell will provide higher quality and more data per run and longer reads (150 bp paired end compared to existing 125 bp paired end).
To keep up with the ever-growing demand for NextSeq, NSC has purchased a second machine and this has been operational since Jan 2016.
With the purchase of the above machines, NSC now has the complete range of Illumina sequencers - HiSeq X, HiSeq 3000/4000, HiSeq 2500, NextSeq 500 and MiSeq available for all types of sequencing solutions!
More about HiSeqX: Illumina HiSeqX
More about HiSeq 4000 and 3000: Illumina HiSeq 4000/3000
The most recent version of our Illumina submission form - v11.1 - was released on Dec 23rd 2015. Please download and use this instead of your old version (under forms tab).
Illumina's latest sequencer model, the NextSeq 500, is now operational at the NSC. With up to 800 million 150 bp reads per 2-day run, the NextSeq bridges the output gap between the HiSeq and MiSeq instruments.
We're now offering Illumina's V4 chemistry as standard on our HiSeqs. That means more reads (150-250 million per end, per lane), and longer reads too - now 125 bp. Perhaps most importantly, run times have been reduced from 12 days to 6, knocking a week off turnaround time.
We are now sequencing several thousand samples per year, and have realised for some time the need for sample tracking software. After extensive validation, we have purchased Genologics Clarity LIMS (http://www.genologics.com/ We will be implementing this over the next months, to ensure your samples are handled in the most secure and timely manner possible.
ForBio (www.forbio.uio.no) announces a course relevant for NSC users: Introduction to Bioinformatics for Biosystematics.
After more than six years of service, the Norwegian Sequencing Centre has decided to phase out one of our first work-horses, the 454 GS FLX. We will complete the sequencing of all the samples we have in the queue, and will accept new samples for 454 sequencing until the 17th of May 2014.
The Norwegian Research Council announced last week that the Norwegian Sequencing Centre (NSC) gets 41 MNOK of funding from 2014.