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The NSC will close over the Christmas break. Deadline for submissions is 4 pm on Wednesday 22nd December. We will reopen again from Monday 3rd January 2022.

God Jul!

Next Generation Cytogenomics has also arrived at the NSC! Optical mapping is a complementary technology to sequencing that enables improved genome assembly and the detection of structural variants (indels, translocations and CNVs) that sequencing struggles to detect. With high coverage of the genome, even low-frequency rearrangements in tumour samples or genetic mosaics can be detected.

 

For early access prior to service launch, or just to learn more, get in touch (post@sequencing.uio.no)!

 

More info at https://bionanogenomics.com

The NSC will be closed in July. The last day for submissions will be Friday 2nd July. We will re-open for submission from August 2nd (OUS) and August 9th (UiO). Projects submitted prior to July 2nd will still be processed over the summer, but turnaround times will be longer. Consulting / advice will only resume when we reopen.

After 5 years of service, we have retired our HiSeq 3000, 4000 and X instruments.  These are superceded by the newer, faster, cheaper NovaSeq. From now, Illumina sequencing will be performed only at the OUS node, and the CEES node will be focusing on long-read PacBio services.

The NSC will close over Easter. Deadline for submissions is 12.00 noon on Wed 31st March. We will reopen again from Tuesday 6th April.

God Påske!

The NSC will close over the Christmas break. Deadline for submissions is 15:00 on Friday 18th December. We will reopen again from Monday 4th January 2021.

God Jul!

We will not be closing over the summer (both UiO and OUS nodes). Depending on demand, we may offer a reduced service. During the month of July, please contact us in advance of making a delivery to ensure we are available to receive your samples on post@sequencing.uio.no

Both the UiO and OUS nodes are open to receive and sequence your samples.

We are not experiencing sickness or shortage of reagents and remain open. However, due to distancing requirements, we have fewer employees on site at any one time, so please ensure we are available to receive your samples before delivery, by mailing us on post@sequencing.uio.no.

We request that all persons delivering samples show consideration, particularly in the hospital environment, and only deliver samples in person if the are:

1. Themselves free of possible COVID-19 symptoms

2. Have no family members in quarantine

 

Both the UiO and OUS nodes are open to receive and sequence your samples.

We are not experiencing sickness or shortage of reagents and remain open. However, due to distancing requirements, we have fewer employees on site at any one time, so please ensure we are available to receive your samples before delivery, by mailing us on post@sequencing.uio.no.

We request that all persons delivering samples show consideration, particularly in the hospital environment, and only deliver samples in person if the are:

1. Themselves free of possible COVID-19 symptoms

2. Have no family members in quarantine

 

Single Cell Sequencing instruments available to use free of charge

We have a Chromium Controller available at both NSC nodes (Blindern and Ullevål). We are not offering 10X single cell library preparation as a service, but make these machines available to users free of charge, as long as you perform the sequencing with us. Single-cell RNA-seq, single-cell ATAC-seq and "linked"  (synthetic long) reads are among the possible applications. To book a machine, please simply inquire on post@sequencing.uio.no.

More info here: https://www.10xgenomics.com/

Our Illumina NovaSeq 6000 system is now available. The latest submission form is updated with all yield options, which range from 400M reads (the same as a HiSeq lane) to 10 Billion reads per flowcell (equivalent to approx 2.5 HiSeq flowcells). We will be happy to help you choose the most appropriate yield from the somewhat confusingly-named flowcells (SP, S1, S2 and S4)!

Run times are all under 48 hours, and 250 bp paired end reads are back in high output on the NovaSeq.  For more information see: https://emea.illumina.com/systems/sequencing-platforms/novaseq/specifications.html.

Users can expect a small drop in price for genome sequencing, but RNA-seq and counting applications such as ChIP-seq will be 15-40% cheaper than before, depending on scale and readlength.

The NSC will close over the Christmas break. Deadline for submissions is 15:00 on Thursday 19th December. We will reopen again from Monday 6th January 2020.

God Jul!

New 16S Service

To meet demand, we have launched a 16S service. We offer amplification and sequencing with the "Fadrosh et al" procedure (https://doi.org/10.1186/2049-2618-2-6), which gives excellent results with only 10% PhiX blend, with sequencing on the MiSeq with 300 bp paired end reads. The V3-V4 regions are targeted with primers 319F-806R. Prices for sample batches of ≤24, ≤48, ≤94 and ≤190 are 35, 40, 45 and 50,000 NOK (including MVA and a MiSeq run). Users must supply purified genomic DNA, and can choose to receive data in FASTQ, QIIME or MOTHUR analysis formats. For input requirements etc., please see our submission guidelines.

10x Genomics Chromium Controller now available at NSC

Single-cell RNA-seq, single-cell ATAC-seq and "linked"  (synthetic long) reads are among the possible applications. If you are interested in accessing these services, please get in touch, as we are soliciting early-access users! More info here: https://www.10xgenomics.com/

After 6 years as a workhorse of the facility, we are now phasing out HiSeq 2500 services. The OUS HiSeq 2500 has been decommissioned, but the machine at CEES will be available until end of March 2019.

The NSC will not be accepting new submissions over the summer break (sequencing of existing submissions will continue, although turnaround times may be longer than usual). Deadline for submissions is 16:00 on Thursday 5th July. We will reopen again from Monday 6th August.

The NSC will not be accepting submissions over Easter. Deadline for submissions is 12.00 noon on Friday 23rd March. We will reopen again from Tuesday 3rd April.

God Påske!

Renovation of the OUS node is complete, and normal services have resumed.

All four HiSeq X machines are now operational! - output capacity at OUS now exceeds 1.5 Tb per day...

 

Pre and post-PCR labs were expanded, and installation of new sample-prep  automation is underway. However, the biggest change is the installation of a powerful air-conditioning system in our enlarged machine room.Two 20 kW systems (one main system, and an independent backup powered from a reserve generator) should ensure stable temperatures in the machine room, improving machine reliabiliy and data quality.

Ask for a quick tour when you drop off your samples!

 

Illumina’s lastest sequencers, HiSeq X, HiSeq 4000 and HiSeq 3000 have now been purchased and are being installed at the NSC.

HiSeq X is targeted at population-scale genome sequencing and NSC now has 4 of these machines. The first has been installed and tested. We expect the remaining 3 machines to be operational before summer.

HiSeq 3000 and 4000 build upon the existing HiSeq 2500 platform using the new HiSeq X patterned flow cell technology. The principle benefit to users will be faster turnaround time (run time is 3 days compared to 6 days in HiSeq 2500). Also, the new patterned flow cell will provide higher quality and more data per run and longer reads (150 bp paired end compared to existing 125 bp paired end).

To keep up with the ever-growing demand for NextSeq, NSC has purchased a second machine and this has been operational since Jan 2016.

With the purchase of the above machines, NSC now has the complete range of Illumina sequencers - HiSeq X, HiSeq 3000/4000, HiSeq 2500, NextSeq 500 and MiSeq available for all types of sequencing solutions!

 

More about HiSeqX: Illumina HiSeqX

More about HiSeq 4000 and 3000: Illumina HiSeq 4000/3000

The most recent version of our Illumina submission form - v11.1 - was released on Dec 23rd 2015. Please download and use this instead of your old version (under forms tab).

Illumina's latest sequencer model, the NextSeq 500, is now operational at the NSC. With up to 800 million 150 bp reads per 2-day run, the NextSeq bridges the output gap between the HiSeq and MiSeq instruments.