Dept. of Medical Genetics Ullevål
Kirkeveien 166 (map)
10x Genomics Chromium Controller now available at NSC
Single-cell RNA-seq, single-cell ATAC-seq and "linked" (synthetic long) reads are among the possible applications. If you are interested in accessing these services, please get in touch, as we are soliciting early-access users! More info here: https://www.10xgenomics.com/
New 16S Service
To meet demand, we have launched a 16S service. We offer amplification and sequencing with the "Fadrosh et al" procedure (https://doi.org/10.1186/2049-2618-2-6), which gives excellent results with only 10% PhiX blend, with sequencing on the MiSeq with 300 bp paired end reads. The V3-V4 regions are targeted with primers 319F-806R. Prices for sample batches of ≤24, ≤48, ≤94 and ≤190 are 35, 40, 45 and 50,000 NOK (including MVA and a MiSeq run). Users must supply purified genomic DNA, and can choose to receive data in FASTQ, QIIME or MOTHUR analysis formats. For input requirements etc., please see our submission guidelines.
The NSC will close over the Christmas break. Deadline for submissions is 15:00 on Thursday 19th December. We will reopen again from Monday 6th January 2020.
After 6 years as a workhorse of the facility, we are now phasing out HiSeq 2500 services. The OUS HiSeq 2500 has been decommissioned, but the machine at CEES will be available until end of March 2019.
Our Illumina NovaSeq 6000 system is now available. The latest submission form is updated with all yield options, which range from 400M reads (the same as a HiSeq lane) to 10 Billion reads per flowcell (equivalent to approx 2.5 HiSeq flowcells). We will be happy to help you choose the most appropriate yield from the somewhat confusingly-named flowcells (SP, S1, S2 and S4)!
Run times are all under 48 hours, and 250 bp paired end reads are back in high output on the NovaSeq. For more information see: https://emea.illumina.com/systems/sequencing-platforms/novaseq/specifications.html.
Users can expect a small drop in price for genome sequencing, but RNA-seq and counting applications such as ChIP-seq will be 15-40% cheaper than before, depending on scale and readlength.