If you want to get information prior to your submission, please use the following inquiry forms below or send us an email at post@sequencing.uio.no
Project enquiry form - to provide the NSC with a description of your proposed sequencing project
HOW TO MAKE A SUBMISSION
Complete the sample submission form (Word file) and sample submission table (Excel file)(if submitting more than 8 samples or a pool) and send it with a gel image (or equivalent) of your samples. When fully complete, send to post@sequencing.uio.no
After document submission please wait for our reply to confirm that everything is complete and correct, then bring or ship your samples to the NorSeq Ullevål node (find address here).
If you need a price quote in order to obtain a purchase order number, contact us to obtain one on post@sequencing.uio.no – do not send an incomplete form and the purchase information later.
Accepted tubes/plates and labeling
Each tube/plate must be marked with the user’s name and date, in addition to sample name. When submitting >16 samples, use 96-well PCR plates, not 8-well stripes or 0.5 ml tubes, (filling in columns – see figure below). Lower numbers of samples should be submitted in 1.5-ml Safelock Eppendorf tubes (please don`t tape Parafilm around the tubes). We do not accept precipitated/dried samples.
Correct layout for filling samples in 96-well PCR plates (i.e. filled by columns from left side, not by rows). Do not leave empty wells or gaps!
Failure to follow these guidelines voids any guarantees on library preparation or run yields!
SAMPLE STORAGE
We will store your submitted samples for a period of 3 months following submission. Samples cannot be returned to users except in exceptional circumstances. We store your completed libraries for at least 1 year following project completion to allow further sequencing if requested.
How to fill the submission form
If your submission consists of more than one sample type (e.g. both DNA for PCR-free library and RNA for miRNA library construction), please complete two separate copies of the submission form.
Section 1: General Sample Information
Enter text/check boxes as required
Section 2: Sample prep requested
Enter text/check boxes as required
Section 3. Sample Information table
Enter text/check boxes as required
Detail the type of sample you are submitting, NOT the prep type you wish performed / have performed.
If combining multiple samples per lane, please indicate which samples may be combined together in the comments field. If you have already performed sample library prep and Illumina-compatible indexing, also detail which index codes (manufacturer, index position in adapter, and index sequence) were used for each sample.
If you have used any whole-genome amplification techniques that attach nucleic acid adapters/linkers to your DNA, provide details in the comments field (including sequence). In addition, if you have used restriction enzyme digestion of your sample, this should be noted here. Both of the above will result in many sequences starting with the same bases, which requires special handling during Illumina sequencing.
Section 4. Sequencing Information Required
If you are unsure/did not receive a recommendation from us as to which kind of sequencing is appropriate, please get in touch with us using the email address below, or complete the project request form available at http://www.sequencing.uio.no/forms/.
The type and length of sequencing have a major effect on the cost of your project, and sequencing of your project cannot begin without this information. Information about the available at NSC Illumina sequencing options can be found here: Illumina sequencers
Please also give a brief description of your project goals, which will help us ensure you get the most appropriate sequencing type and length. Norwegian projects involving human subjects are required to provide a REK number.
Section 5. Data Delivery:
Please select your preferred data delivery method. For non-sensitive data, it is usually convenient to select a download option. Sensitive data can only be delivered on hard drives or through secure networks. If you have a secure network available to which we may upload the data, please contact us to arrange access.
Section 6. Contact, Billing & Delivery details (All fields mandatory):
We require a single person to act as our contact regarding sample preparation. This person must also be capable of providing information on billing & data delivery, but you are required also to enter the name of the relevant purchasing official to whom the bill should be addressed. SUBMISSIONS WITHOUT A VALID PURCHASE ORDER NUMBER WILL BE REJECTED. Should you require an estimate in order to generate this number, please get in touch with us at the address below. If your institution does not use an order number system, then please enter “not applicable”.
Note that additional information is mandatory for internal OUS orders.
Registering us as a service provider: The NSC is not a legal entity, and you will therefore not find us in the Brønnøysund Register. Your invoice will be sent from either Oslo University Hospital or the University of Oslo, Banking details can be provided if necessary.
Pricing: Our prices vary in accordance with USD:NOK exchange rates at the time reagents are purchased for your project. Please contact us for an estimate for your project. We provide quotes, usually valid for 14 days, which you can use to raise a purchase order number for your submission.
Section 7. Sample Delivery Information
Please submit the completed submission form by email prior to making your delivery. During seasonal holiday periods, we may not accept submissions – please check www.sequencing.uio.no for news prior to sending samples. Provide carrier information, or if delivering in person, please check that we are available to receive you.
DO NOT send samples by registered post or TNT carrier (they may be delayed for several days in internal post before we are notified that our signature is required to collect the package).
Deliver/send samples to:
NSC Ullevål node/Gregor Gilfillan
Medisinsk Genetikk,
Lab. bygget 2. etasje (Nord)
Oslo Universitetssykehus (Ullevål)
Kirkeveien 166
0450 Oslo
Norway
A map can be found here: http://www.sequencing.uio.no/contact/
Tel: +47 23 01 64 19
Email: ous-seq@sequencing.uio.no