We offer Illumina sequencing on Novaseq X, NextSeq 500 , MiSeq i100 and MiSeq instruments. Find specifications for the Illumina sequencing platforms below.
MiSeq (Link to Illumina specs: Miseq)
Applications: Small genome DNA sequencing (de novo & resequencing), Metagenomics studies (including rDNA), Amplicon sequencing, Targeted resequencing such as gene panels, Control of libraries for large projects, smallRNA/microRNA sequencing, Epigenetic studies with few samples.
| Sequencer/Run type | Read length | Output per run | NSC guarantee | |
| reads per end (M) | Bases (Gb) | reads per end (M) | ||
| MiSeq v3 | 75 PE | 22 - 25 | 3.3 - 3.8 | 10* |
| 300 PE | 13 - 15 | |||
| Miseq v2 | 50 SR | 12 - 15 | 0.7 - 0.8 | 6* |
| 25 PE | 0.7 - 0.8 | |||
| 150 PE | 4.5 - 5.1 | |||
| 250 PE | 7.5 - 8.5 | |||
| MiSeq v2 Micro | 150 PE | 4 | 1.2 | 2* |
| MiSeq v2 Nano | 150 PE | 1 | 0.3 | 0.5* |
| 250 PE | 0.5 | |||
MiSeq i100 (Link to Illumina specs: MiSeq i100)
The MiSeq i100 is primarily used for small- to medium-scale sequencing projects that require fast turnaround and high accuracy. Its main applications include targeted sequencing (such as amplicon and gene panels), small genome sequencing (bacteria, viruses, plasmids), and mitochondrial DNA sequencing. It is also commonly used for amplicon-based metagenomics (e.g. 16S/ITS) and small RNA-seq or pilot transcriptomics studies. Can also be used as a control of libraries or pooling for larger projects.
| Flow cell | Read length | Output per run | NSC guarantee | |
| reads per end (M) | Bases (Gb) | reads per end (M) | ||
| 5M | 150 PE | 5 | 1.5 | 2.5* |
| 300 PE | 3 | |||
| 25M | 100 SR / 50 PE | 25 | 2.5 | 12.5* |
| 150 PE | 7.5 | |||
| 300 PE | 15 | |||
| 500 PE | 25 | |||
| 50M | 100 SR / 50 PE | 50 | 5 | 25* |
| 150 PE | 15 | |||
| 300 PE | 30 | |||
| 100M | 100 SR/50 PE | 100 | 10 | 50* |
| 150 PE | 30 | |||
NextSeq 500 (Link to Illumina specs: NextSeq)
Applications: Genomic DNA sequencing, Exome sequencing, mRNA sequencing, smallRNA/microRNA sequencing, Epigenetics, ChIP-seq, DNA methylation, Small genome DNA sequencing (de novo & resequencing), Targeted resequencing such as gene panels.
| Sequencer/Run type | Read length | Output per run | NSC guarantee | |
| reads per end (M) | Bases (Gb) | reads per end (M) | ||
NextSeq High Output | 75 SE | 400 | 25 - 30 | 200* |
| 75 PE | 50 - 60 | |||
| 150 PE | 100 - 120 | |||
NextSeq Mid Output | 75 PE | 130 | 16 - 19 | 65* |
| 150 PE | 32 - 39 | |||
NovaSeq X (Link to Illumina specs: NovaSeq X)
The NovaSeq X flowcells 10B and 25B can be split into 8 separate lanes, and the 1.5B flowcell into 2 lanes. We will run half or 1/8 flow cells only when we have enough other projects to fill the flow cell, but are running the 1.5B and 25B, read length 150 bp PE, on a regular basis.
Applications: Genomic DNA sequencing, Metagenomics, Exome sequencing, mRNA sequencing, smallRNA/microRNA sequencing, Epigenetics, ChIP-seq, DNA methylation.
Flow cell | Read length | Output perm run | NSC guarantee | |
| reads per end (M) | Bases (Gb) | reads per end (M) | ||
NovaSeq X 1.5B
| 50 PE | 1600 (800 per lane) | 165 | 800* (400 per lane) |
| 100 PE | 330 | |||
| 150 PE | 500 | |||
| 300 PE | 1 000 | |||
| NovaSeq x 10B | 50 PE | 10 000 (1 200 per lane) | 1 000 | 5000* (600 per lane) |
| 100 PE | 2 000 | |||
| 150 PE | 3 000 | |||
| NovaSeq X 25B | 50 PE | 26 000 (3 250 per lane) | 2 500 | 13 000* (1 600 per lane) |
| 100 PE | 5 500 | |||
| 150 PE | 8 000 | |||
Data output performance guarantees
If your samples are submitted in accordance with our guidelines and meet our quality and quantity requirements, we provide the performance guarantees given in the table. Note that these are our minimal output criteria and we routinely expect greater output. Should we fail to meet these targets, we will run additional sequencing at no cost to you or offer a discount as appropriate. In the case of multiplexed samples, we cannot guarantee equal distribution of reads between different indexes, but we do run a qPCR assay to achieve this as best possible.
*Exceptions: Data guarantees are not valid for long-insert libraries (insert size > 500 bp) and low-diversity samples (e.g. PCR amplicons, restriction digestion fragments, RAD libraries), which require dilution and blending with control library using Illumina technology.
Note that if your submitted samples do not meet our quality or quantity criteria, we may still be willing to attempt sample prep and sequencing, but our performance guarantee no longer applies and you will be liable for all expenses incurred regardless of the output amount, also in the event that no sequence data can be generated.
PhiX blending
It is standard practice to spike Illumina sequencing runs with 1% PhiX library, which acts as a reference for sequencing performance. The level of PhiX blend must be increased for low-diversity samples, up to 50%, at the expense of yield. If you are concerned that the use of PhiX will cause problems for your downstream data analysis, it is your responsibility to tell us this and request an alternative solution.