HiSeq 2500


Run parameters, high output mode (2 flow cells, each with 8 lanes available for user samples):

  • Up to 4 billion reads (8 billion paired end) per dual flow-cell run
  • Single-read or paired-end
  • Read lengths of 50 or 125 bp
  • Total output up to 1 Tbp per run. Run durations up to 6 days. 


Additional Run parameters, rapid run mode

In addition to the parameters above, the 2500 instrument can also be run in a "rapid" mode that offers lower throughput but at greater speed. This also allows longer read length (all at increased cost per base compared to the regular runs).

  • Up to 600 million reads (1.2 billion paired end)
  • Read lengths up to 150 bp
  • Total output up to 180 Gbp per run. Run duration approx. 40 h.


Applications include:

  • Genomic DNA sequencing
  • Exome sequencing
  • mRNA sequencing
  • smallRNA/microRNA sequencing
  • Epigenetics
    • Chromatin immunoprecipitation sequencing
    • DNA methylation


Most samples can be run as either Paired-End (PE) or Single-Read (SR) experiments

Published Oct. 27, 2016 12:13 PM - Last modified Oct. 27, 2016 1:12 PM