Illumina sequencers available at NSC

 

MiSeq (Link to Illumina specs: Miseq)

Applications: Small genome DNA sequencing (de novo & resequencing); Metagenomics studies (including rDNA); Amplicon sequencing; Targeted resequencing, such as gene panels; Control of libraries for large projects; smallRNA/microRNA sequencing; Epigenetic studies with few samples

Sequencer Read length Output per run NSC guarantee
reads per end (M) Bases (Gb) reads per end (M)
MiSeq v3 75 PE 22 - 25 3.3 - 3.8 10*
300 PE 13 - 15
Miseq v2

36 PE

12 - 15 0.5 - 0.6 6*
25 PE 0.7 - 0.8
150 PE 4.5 - 5.1
250 PE 7.5 - 8.5
MiSeq v2 micro 150 PE 4 1.2 2*
MiSeq v2 nano 150 PE 1 0.3 0.5*
250 PE 0.5

 

NextSeq 500 (Link to Illumina specs: NextSeq)

Applications: Genomic DNA sequencing; Exome sequencing; mRNA sequencing; smallRNA/microRNA sequencing; Epigenetics: Chromatin immunoprecipitation sequencing; DNA methylation; Small genome DNA sequencing (de novo & resequencing); Targeted resequencing, such as gene panels

Sequencer Read length Output per run NSC guarantee
reads per end (M) Bases (Gb) reads per end (M)

NextSeq High

Output

75 SE 400 25 - 30 200*
75 PE 50 - 60
150 PE 100 - 120

NextSeq Mid

Output

75 PE 130 16 - 19 65*
150 PE 32 - 39

 

HiSeq 3/4000 & HiSeq X (Link to Illumina specs: HiSeq 3/4000, HiSeqX) Each run contains 8 separate lanes

Applications: Genomic DNA sequencing (the only application for HiseqX); Exome sequencing; mRNA sequencing; smallRNA/microRNA sequencing; Epigenetics: Chromatin immunoprecipitation sequencing, DNA methylation

Sequencer Read length Output per run NSC guarantee

reads per end (M)

Bases (Gb) reads per end (M)
HiSeq 3/4000 50 SE 2500 105 - 125 1250*
75 PE 325 - 375
150 PE 650 - 750
HiSeq X 150 PE

5300 - 6000

800 - 900 2500*

 

NovaSeq (Link to Illumina specs: NovaSeq)

With NovaSeq Xp reagents, we can separate the SP, S1 and S2 flow cells into 2 separate lanes, S4- into 4 lanes. We will run half or quarter flow cells only when we have enough other projects to fill the flow cell (longer queue times).

Applications: Genomic DNA sequencing; Metagenomics; Exome sequencing; mRNA sequencing; smallRNA/microRNA sequencing; Epigenetics: Chromatin immunoprecipitation sequencing, DNA methylation

Sequencer Read length Output per run NSC guarantee
reads per end (M) Bases (Gb) reads per end (M)
NovaSeq SP 50 PE 650 - 800 65 - 80 400*
100 PE 134 - 167
150 PE 200 - 250
250 PE 325 - 400
NovaSeq S1

50 PE

1300 - 1600 134 - 167 800*
100 PE 266 - 333
150 PE 400 - 500
NovaSeq S2 50 PE 3300 - 4100 333 - 417 2000*
100 PE 667 - 833
150 PE 1000 - 1250
NovaSeq S4 100 PE 8000 - 10 000 1600 - 2000 5000*
150 PE 2400 - 3000

 

Data output performance guarantees

If your samples are submitted in accordance with these guidelines and meet our quality and quantity requirements, we provide the performance guarantees given in the table. Note that these are our minimal output criteria and we routinely expect greater output. Should we fail to meet these targets, we will run additional sequencing at no cost to you or offer a discount as appropriate. In the case of multiplexed samples, we cannot guarantee equal distribution of reads between different indexes, but we do run a qPCR assay to achieve this as best possible.

*Exceptions: Data guarantees are not valid for long-insert libraries (insert size > 500 bp) and low-diversity samples (e.g. PCR amplicons, restriction digestion fragments, RAD libraries), which require dilution and blending with control library using Illumina technology.

Note that if your submitted samples do not meet our quality or quantity criteria, we may still be willing to attempt sample prep and sequencing, but our performance guarantee no longer applies and you will be liable for all expenses incurred regardless of the output amount, also in the event that no sequence data can be generated.

Published Mar. 24, 2020 4:07 PM - Last modified Apr. 6, 2020 9:12 AM