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The NSC will close over Easter. Deadline for submissions is 2 pm on Tuesday 26.03. We will reopen again from Tuesday 02.04.

God Påske!

The Ullevål sequencers generated more than 1 petabase (1015 bp) in 2023. This is equivalent to sequencing 12,000 human genomes at 30x. The NSC handled more than 20,000 samples across 300+ projects during the year. 

Look out for the live base-counter screen (pictured) when dropping off your samples!

The NSC will close 3 pm on Thursday 21st December. We will reopen again from Tuesday 2nd January 2024.

God Jul!

New single cell/nuclei suspension automation from S2 Genomics available to use free of charge

To assist single-cell studies, users can now access the S2 Singulator-100 instrument at the NSC Ullevål node. Using disposable cartridges, the Singulator dissociates tissue samples into single cell or nuclei suspensions by combined mechanical agitation and enzymatic digestion. The output is a pre-filtered suspension that can be further processed for single-cell sequencing. Automation of the dissociation process saves time and is more reproducible than manual processing.  The machine is available for use free of charge, as long as you perform the sequencing with us afterwards (cartridges and reagents must be purchased). For more information, see S2 Genomics - Singulator 100, and contact us on post@sequencing.uio.no for an introduction and to reserve the machine.

 

PacBio's newest instrument has successfully been installed in our lab. 

With a high-density, 25 million ZMW SMRT Cell, up to 4 SMRT Cells in parallel, and 24-hour run times, the Revio system delivers 360 Gb of HiFi reads per day. Compared to Sequel IIe, Revio gives 15× increase in HiFi read throughput.

We expect to offer long-read sequencing on Revio from August 2023. Please do not hesitate to contact us already now for information about pricing and DNA requirements!

 


The NSC will offer a reduced service over summer. New projects will not be accepted in July. Projects already queued will be sequenced, but longer turnaround times should be expected. Deadline for submissions is 12 noon on Friday 30th June. Submissions and regular services resume from Tuesday 1st August.

God Sommer!


The NSC will close over Easter. Deadline for submissions is 3 pm on Friday 31.03. We will reopen again from Tuesday 11.04.

God Påske!

The NSC will close 3 pm on Thursday 22nd December. We will reopen again from Tuesday 3rd January 2023.

God Jul!

New Single Cell Sequencing instruments from Mission Bio available to use free of charge

Expanding our single-cell offerings, users can now access Mission Bio's technologi at the NSC Ullevål node. The Tapestri instrument enables targeted re-sequencing of single cells, such as detection of SNPs and CNVs at the single-cell level. Applications include characterising tumour heterogeneity , checking off-target hits in genome-editing, or measuring levels of mosaicism. Single-cell DNA methylation analysis is in development. These capabilities can be combined with oligo-tagged antibodies to detect the co-expression of proteins with SNPs / CNVs (single-cell multi-omics). The machine is available for use free of charge, as long as you perform the sequencing with us afterwards! For more information, see https://missionbio.com/, and contact us on post@sequencing.uio.no for an introduction and to reserve the machine.

 

After the digital PacBio Day in 2021, we are happy to announce that this year we plan for in-person event in the campus! We will also expand it to a full-day event and include human research.

Stay tuned for more information and registration later this summer!

The NSC will close over summer. Deadline for submissions is 3 pm on Thursday 30th June. Projects queued before this deadline will still be processed over summer, but longer turnaround times should be expected. We reopen again from Monday 1st August.

The NSC will close over Easter. Deadline for submissions is 3 pm on Tuesday 12th of April. We will reopen again from Tuesday 19th April.

God Påske!

The NSC will close over the Christmas break. Deadline for submissions is 4 pm on Wednesday 22nd December. We will reopen again from Monday 3rd January 2022.

God Jul!

Next Generation Cytogenomics has also arrived at the NSC! Optical mapping is a complementary technology to sequencing that enables improved genome assembly and the detection of structural variants (indels, translocations and CNVs) that sequencing struggles to detect. With high coverage of the genome, even low-frequency rearrangements in tumour samples or genetic mosaics can be detected.

 

For early access prior to service launch, or just to learn more, get in touch (post@sequencing.uio.no)!

 

More info at https://bionanogenomics.com

The NSC will be closed in July. The last day for submissions will be Friday 2nd July. We will re-open for submission from August 2nd (OUS) and August 9th (UiO). Projects submitted prior to July 2nd will still be processed over the summer, but turnaround times will be longer. Consulting / advice will only resume when we reopen.

After 5 years of service, we have retired our HiSeq 3000, 4000 and X instruments.  These are superceded by the newer, faster, cheaper NovaSeq. From now, Illumina sequencing will be performed only at the OUS node, and the CEES node will be focusing on long-read PacBio services.

The NSC will close over Easter. Deadline for submissions is 12.00 noon on Wed 31st March. We will reopen again from Tuesday 6th April.

God Påske!

The NSC will close over the Christmas break. Deadline for submissions is 15:00 on Friday 18th December. We will reopen again from Monday 4th January 2021.

God Jul!

We will not be closing over the summer (both UiO and OUS nodes). Depending on demand, we may offer a reduced service. During the month of July, please contact us in advance of making a delivery to ensure we are available to receive your samples on post@sequencing.uio.no

Both the UiO and OUS nodes are open to receive and sequence your samples.

We are not experiencing sickness or shortage of reagents and remain open. However, due to distancing requirements, we have fewer employees on site at any one time, so please ensure we are available to receive your samples before delivery, by mailing us on post@sequencing.uio.no.

We request that all persons delivering samples show consideration, particularly in the hospital environment, and only deliver samples in person if the are:

1. Themselves free of possible COVID-19 symptoms

2. Have no family members in quarantine

 

Both the UiO and OUS nodes are open to receive and sequence your samples.

We are not experiencing sickness or shortage of reagents and remain open. However, due to distancing requirements, we have fewer employees on site at any one time, so please ensure we are available to receive your samples before delivery, by mailing us on post@sequencing.uio.no.

We request that all persons delivering samples show consideration, particularly in the hospital environment, and only deliver samples in person if the are:

1. Themselves free of possible COVID-19 symptoms

2. Have no family members in quarantine

 

Single Cell Sequencing instruments available to use free of charge

We have a Chromium Controller available at both NSC nodes (Blindern and Ullevål). We are not offering 10X single cell library preparation as a service, but make these machines available to users free of charge, as long as you perform the sequencing with us. Single-cell RNA-seq, single-cell ATAC-seq and "linked"  (synthetic long) reads are among the possible applications. To book a machine, please simply inquire on post@sequencing.uio.no.

More info here: https://www.10xgenomics.com/

Our Illumina NovaSeq 6000 system is now available. The latest submission form is updated with all yield options, which range from 400M reads (the same as a HiSeq lane) to 10 Billion reads per flowcell (equivalent to approx 2.5 HiSeq flowcells). We will be happy to help you choose the most appropriate yield from the somewhat confusingly-named flowcells (SP, S1, S2 and S4)!

Run times are all under 48 hours, and 250 bp paired end reads are back in high output on the NovaSeq.  For more information see: https://emea.illumina.com/systems/sequencing-platforms/novaseq/specifications.html.

Users can expect a small drop in price for genome sequencing, but RNA-seq and counting applications such as ChIP-seq will be 15-40% cheaper than before, depending on scale and readlength.

The NSC will close over the Christmas break. Deadline for submissions is 15:00 on Thursday 19th December. We will reopen again from Monday 6th January 2020.

God Jul!