ChIP DNA library preps

Prep. type Min. amount Recommend amount Max. volume Accepted buffers

ChIP / low-input DNA

0.1 ng 1 - 10 ng

20 μl

5 - 10 mM Tris

Input requirements for ChIP DNA library preps.

Submission:

It is not necessary to provide gel or Bioanalyzer documentation of ChIP sample size or quality, nor 260/280 or 260/230 ratios. However, it is necessary to document the size of input material used prior to immunoprecipitation.

If possible, please provide a concentration of the ChIP sample determined by a fluorometric method such as Qubit, as this will greatly increase your chances of obtaining a high-quality sequencing library. Do not use a Nanodrop instrument.

Note that ChIP samples must not contain salmon sperm DNA or other nucleic acid blocking agents.

Published Mar. 20, 2020 5:08 PM - Last modified Mar. 30, 2020 2:14 PM