ChIP DNA library preps
|Prep. type||Min. amount||Recommend amount||Max. volume||Accepted buffers|
ChIP / low-input DNA
|0.1 ng||1 - 10 ng||
5 - 10 mM Tris
Input requirements for ChIP DNA library preps.
It is not necessary to provide gel or Bioanalyzer documentation of ChIP sample size or quality, nor 260/280 or 260/230 ratios. However, it is necessary to document the size of input material used prior to immunoprecipitation.
If possible, please provide a concentration of the ChIP sample determined by a fluorometric method such as Qubit, as this will greatly increase your chances of obtaining a high-quality sequencing library. Do not use a Nanodrop instrument.
Note that ChIP samples must not contain salmon sperm DNA or other nucleic acid blocking agents.