Small (micro) RNA library preps

Prep. type Min. amount Recommend amount Max. volume Accepted buffers

 

Small

RNA

0.2 μg total RNA

or

Purified small RNAs (preferred)

2 - 5 μg total RNA

or

Purified from 2 - 5 μg starting total RNA

10 μl

 

10 μl

H20

 

H20

Input requirements for small (micro) RNA library preps.

Total RNA must be prepared by a method that retains small RNAs. Various vendors provide kits and protocols with adaptations to retain small RNAs. Avoid precipitating RNA, as this reduces the content of small RNAs.

Due to large variations in miRNA amounts in different tissues, success of miRNA preps is not guaranteed. Users submitting total RNA will therefore be billed for reactions consumed even in the event we are unable to prepare libraries. In the event that preps from total RNA are unsuccessful, users may attempt to enrich the small RNA fraction by PAGE or column fractionation and submit this instead, but be sure to note this in the submission form.

Published Mar. 20, 2020 4:13 PM - Last modified Mar. 30, 2020 2:09 PM