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The NSC will close over the Christmas period. The last day for submissions in 2017 will be Thursday 21st December. We will reopen again from 3rd January.
Renovation of the OUS node is complete, and normal services have resumed.
All four HiSeq X machines are now operational! - output capacity at OUS now exceeds 1.5 Tb per day...
Pre and post-PCR labs were expanded, and installation of new sample-prep automation is underway. However, the biggest change is the installation of a powerful air-conditioning system in our enlarged machine room.Two 20 kW systems (one main system, and an independent backup powered from a reserve generator) should ensure stable temperatures in the machine room, improving machine reliabiliy and data quality.
Ask for a quick tour when you drop off your samples!
The OUS node of the NSC will perform renovations during the period 5th October - 4th November. During this time, the OUS node will be unable to perform sample prep or run sequencing.
- HiSeq 4000 sequencing and sample prep services at the UiO node are unaffected.
- No MiSeq, NextSeq or Hiseq X (machines located at OUS) sequencing will be possible during this time.
- The OUS node will continue to accept submissions over this period, which will be queued on a first-come-first-served basis as usual. However, work on the samples can not start until we reopen.
Illumina’s lastest sequencers, HiSeq X, HiSeq 4000 and HiSeq 3000 have now been purchased and are being installed at the NSC.
HiSeq X is targeted at population-scale genome sequencing and NSC now has 4 of these machines. The first has been installed and tested. We expect the remaining 3 machines to be operational before summer.
HiSeq 3000 and 4000 build upon the existing HiSeq 2500 platform using the new HiSeq X patterned flow cell technology. The principle benefit to users will be faster turnaround time (run time is 3 days compared to 6 days in HiSeq 2500). Also, the new patterned flow cell will provide higher quality and more data per run and longer reads (150 bp paired end compared to existing 125 bp paired end).
To keep up with the ever-growing demand for NextSeq, NSC has purchased a second machine and this has been operational since Jan 2016.
With the purchase of the above machines, NSC now has the complete range of Illumina sequencers - HiSeq X, HiSeq 3000/4000, HiSeq 2500, NextSeq 500 and MiSeq available for all types of sequencing solutions!
More about HiSeqX: Illumina HiSeqX
More about HiSeq 4000 and 3000: Illumina HiSeq 4000/3000
The most recent version of our Illumina submission form - v11.1 - was released on Dec 23rd 2015. Please download and use this instead of your old version (under forms tab).
Illumina's latest sequencer model, the NextSeq 500, is now operational at the NSC. With up to 800 million 150 bp reads per 2-day run, the NextSeq bridges the output gap between the HiSeq and MiSeq instruments.
We're now offering Illumina's V4 chemistry as standard on our HiSeqs. That means more reads (150-250 million per end, per lane), and longer reads too - now 125 bp. Perhaps most importantly, run times have been reduced from 12 days to 6, knocking a week off turnaround time.
We are now sequencing several thousand samples per year, and have realised for some time the need for sample tracking software. After extensive validation, we have purchased Genologics Clarity LIMS (http://www.genologics.com/ We will be implementing this over the next months, to ensure your samples are handled in the most secure and timely manner possible.
ForBio (www.forbio.uio.no) announces a course relevant for NSC users: Introduction to Bioinformatics for Biosystematics.
After more than six years of service, the Norwegian Sequencing Centre has decided to phase out one of our first work-horses, the 454 GS FLX. We will complete the sequencing of all the samples we have in the queue, and will accept new samples for 454 sequencing until the 17th of May 2014.
The Norwegian Research Council announced last week that the Norwegian Sequencing Centre (NSC) gets 41 MNOK of funding from 2014.